Lung inflammation and irritation
Activation of dendritic cells
Our in vitro alveolar model can discriminate respiratory sensitizers from irritants based on the increased expression of at least one of the three cell surface markers, TSLPr, CD54 and CD86.
The model correctly identifies the pro-haptens Ethylenediamine (EDA) and Piperazine (Pip) as respiratory sensitizers, chemicals which have been reported as false negative in other assays.
The cell viability and the increased expression of the evaluated cell surface markers on DC-THP-1 cells demonstrates the capacity of the in vitro model to mimic the complexity of the alveolar barrier.
Definition of respiratory irritation/sensitization markers
Panel of cytokines able to identify respiratory sensitizers
Possibility to discriminate between irritants and sensitizers
* Chary, A., Serchi, T., Moschini, E., Hennen, J., Cambier, S., Ezendam, J., Blömeke, B., Gutleb, A.C. 2019.